Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Validation of a duplex PCR technique using the gen E and RNase P for the diagnosis of SARS-CoV-2Article Published on 2022-10-012022-11-16 Journal: Enfermedades infecciosas y microbiologia clinica ( [Category] COVID19(2023년), SARS, 치료기술, [키워드] Analysis Berlin protocol Charité clinical samples COVID-19 COVID-19 diagnosis Cribado masivo Diagnosis diagnosis of SARS-CoV-2 Diagnóstico E gene English Faculty of Medicine Genes independent Laboratory limit of detection limitations mass Mass screening no significant difference not affect PCR PCR en tiempo real polymerase chain protocol reagent real time Real-time PCR reduce reliability reported reproducibility reverse transcriptase RNA sample RNase P RT-PCR SARS-CoV-2 SARS-CoV-2. sensitivity Sensitivity and specificity similarity Spanish specificity specimen threshold validation World Health Organization [DOI] 10.1016/j.eimce.2022.05.005 PMC 바로가기
Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern진화하는 바이러스에 대한 진화하는 전략: 우려되는 모든 SARS-CoV-2 변이체를 검출하기 위한 실시간 PCR 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] Alpha Amino acid amino acid change amino acid changes approach B.1.1.7 Beta Canada characterized clinical sensitivity clinical specificity complementary COVID-19 Critical cross-reactivity Delta detect development discriminatory mutation E gene E484K FIVE Gamma Genome sequencing implementation K417N L452R less limit of detection Lineage molecular MONITOR N501Y Negative predictive value nucleotide omicron Omicron variants P681 Pathogens PCR PCR assays positive Positive predictive value positive predictive values positive sample predictive values reaction real-time PCR assay real-time reverse transcriptase real-time RT-PCR replacement respiratory respiratory pathogen responsible reverse transcriptase rRT-PCR SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genome sequencing SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Single nucleotide polymorphism single nucleotide polymorphisms specificity Spike protein subset Surveillance target the SARS-CoV-2 Variability variant variants variants of concern variants of concern. VoC VOCs while wild-type Δ69-70 [DOI] 10.1016/j.jviromet.2022.114553 PMC 바로가기 [Article Type] Article
Anti-factor H antibody associated hemolytic uremic syndrome following SARS-CoV-2 infectionSARS-CoV-2 감염 후 항인자 H 항체 관련 용혈성 요독 증후군Case Reports Published on 2022-09-012022-09-11 Journal: Pediatric Nephrology (Berlin, Germany) [Category] MERS, SARS, 진단, 치료기술, [키워드] Abnormalities Abstract Alternative complement pathway antibody Atypical autoantibody CFHR1 Chain Reaction complement Complement factor H related protein consecutive patient Coronavirus 19 cut-off Cyclophosphamide dialysis disease episode of estimated glomerular filtration rate Factor Factor H febrile illness filtration rate Follow-up Genetic Genetic studies glomerular filtration rate hemolytic uremic syndrome homozygous Increased information initial management median median titer mycophenolate mycophenolate mofetil New one patient pandemic Pathogenesis Patient patients plasma plasma exchanges polymerase chain polymerase chain reaction Prednisolone ranged required respiratory respiratory symptom respiratory symptoms reverse transcriptase Reverse transcriptase polymerase chain reaction RT-PCR SARS-CoV-2 SARS-COV-2 infection screened serology Specific thrombotic microangiopathy Thrombotic microangiopathy. transcriptase Trigger triggered uremic syndrome [DOI] 10.1007/s00467-021-05390-4 PMC 바로가기 [Article Type] Case Reports
The prognostic value of biomarker levels and chest imaging in patients with COVID-19 presenting to the emergency department응급실에 제출하는 COVID-19 환자의 바이오마커 수치 및 흉부 영상의 예후 가치Article Published on 2022-09-012022-09-11 Journal: The American journal of emergency medicine [Category] COVID19(2023년), MERS, SARS, 바이오마커, [키워드] 95% CI age AUC Biomarker Biomarkers C-reactive protein Care Chain Reaction Chest CT chest imaging COVID-19 COVID-19 patient COVID-19 patients CRP D-dimer Department dimer Emergency emergency department ferritin Hospital mortality ICU admission in-hospital mortality Laboratory parameters Lactate lactate dehydrogenase LDH Logistic regression Lung imaging lymphocyte mechanical ventilation Mortality Natriuretic neutrophil Neutrophil to lymphocyte ratio. neutrophil/lymphocyte ratio NLR not performed obesity Patient patients with COVID-19 peptide performed polymerase chain polymerase chain reaction positive presenting Primary outcome procalcitonin prognostic Prognostic value Quantitative real-time reverse transcriptase retrospective reverse transcriptase Reverse transcriptase polymerase chain reaction severity significantly tested troponin vital sign [DOI] 10.1016/j.ajem.2022.06.043 PMC 바로가기 [Article Type] Article
Evaluation of a new “all in one” SARS-CoV-2 antigen-detecting rapid diagnostic test and self-test: Diagnostic performance and usability in child and adult populations새로운 "올인원" SARS-CoV-2 항원 검출 신속 진단 테스트 및 자가 테스트 평가: 어린이 및 성인 인구의 진단 성능 및 유용성Multicenter Study Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Adults age Antigen antigen testing Chain Reaction children children COVID testing classic COVID COVID-19 diagnostic diagnostic test diagnostic testing diagnostic tests Epidemics evaluate evaluated faster Health health priorities investigated less majority multicenter multicenter study multiplex nasal Nasal sampling nasal swab nasopharyngeal sample Nasopharyngeal samples Other respiratory viruses participant performed Perspective point-of-care polymerase chain polymerase chain reaction Population Rapid diagnostic test respiratory infections respiratory viruses reverse transcriptase Reverse transcriptase polymerase chain reaction risk RT-PCR Saliva SARS-CoV-2 Self-test specificity switching transcriptase trauma Usability usability. [DOI] 10.1002/jmv.27812 PMC 바로가기 [Article Type] Multicenter Study
Managing an evolving pandemic: Cryptic circulation of the Delta variant during the Omicron rise진화하는 전염병 관리: 오미크론 상승 중 Delta 변종의 비밀 순환Article Published on 2022-08-252022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Chain Reaction Characteristics circulation decrease Delta delta variant diminishe dominant eliminated Epidemiology expected Increases indicated initial maintain managing morbidity Mutation omicron Omicron variant pandemic polymerase chain polymerase chain reaction Quantitative real time reverse transcriptase Reverse transcriptase quantitative polymerase chain reaction RT-qPCR SARS-CoV-2 side Spread Susceptible-infected-recovered model variant variants variants of concern Wastewater-based epidemiology. [DOI] 10.1016/j.scitotenv.2022.155599 PMC 바로가기 [Article Type] Article
Simultaneous monitoring of eight human respiratory viruses including SARS-CoV-2 using liquid chromatography-tandem mass spectrometry액체 크로마토그래피-탠덤 질량분석기를 이용한 SARS-CoV-2를 포함한 8종의 인간 호흡기 바이러스 동시 모니터링Article Published on 2022-08-042022-09-11 Journal: Scientific Reports [Category] SARS, 신종인플루엔자, 진단, 치료기술, 치료제, [키워드] 229E absence acute infection acute respiratory syndrome coronavirus applied Chain Reaction characterisation clinical sample clinical samples conditions coronavirus 2 Coverage Culture Diagnosis eight H1N1 H3N2 human respiratory virus Infection Influenza Influenza A/H1N1 influenza B information liquid Liquid chromatography mass spectrometry moderate not used nucleoprotein OC43 Orf9b peptide peptides polymerase chain polymerase chain reaction positive presence or absence Protein proteomics provide Replication respiratory respiratory virus respiratory viruses reverse transcriptase Reverse transcriptase polymerase chain reaction RT-PCR SARS-CoV-2 SARS-CoV-2 ORF9b serology Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Simultaneous specimens Tandem mass spectrometry the SARS-CoV-2 transcriptase viral infection Viral load Viral protein Viral proteins Viral RNA virus virus replication [DOI] 10.1038/s41598-022-16250-y PMC 바로가기 [Article Type] Article
Preoperative SARS-CoV-2 Screening Fails to Detect Viral Particles Prior to Airway Surgery수술 전 SARS-CoV-2 스크리닝은 기도 수술 전에 바이러스 입자를 감지하지 못함Article Published on 2022-08-012022-09-11 Journal: The Laryngoscope [Category] SARS, 치료기술, [키워드] adenoid adenoidectomy adenoidectomy. Asymptomatic asymptomatic patient asymptomatic patients Care Chain Reaction Child children collected conducted convenience sampling Course COVID COVID-19 cross-sectional study effect size Evidence evidence of Fail Infection Laryngoscope Level Logistic regression Nasopharyngeal swab nucleic acid Odds ratio Older Particle Patient pediatric polymerase chain reaction positive preoperative Prospective Study protocol question real-time reverse transcription-polymerase chain reaction reverse transcriptase SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 particle SARS-CoV-2 particles SARS-CoV-2 viral particle SARS-CoV-2 viral particles Screening specimens surgery surgical These data tissue Total utility variable viral particle Viral particles Viral transmission was used [DOI] 10.1002/lary.29906 PMC 바로가기 [Article Type] Article
Evaluation of a fully closed real time PCR platform for the detection of SARS-CoV-2 in nasopharyngeal swabs: a pilot study비인두 면봉에서 SARS-CoV-2 검출을 위한 완전 폐쇄형 실시간 PCR 플랫폼 평가: 파일럿 연구Article Published on 2022-08-012022-09-11 Journal: Journal of Clinical Pathology [Category] SARS, 치료기술, [키워드] addition analysed approach approach clinical follow-up close contact contact with COVID-19 cycle threshold demonstrated develop Diagnosis diagnosis of COVID-19 evaluate FIVE Follow-up gold gold standard infection control infection control. initial nasopharyngeal Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nasopharyngeal swabs Patient PCR pilot study platform positive positive result real time retrieved reverse transcriptase reverse transcriptase PCR robust RT-PCR RT-PCR test SARS-CoV-2 SARS-COV-2 infection sensitivity Sensitivity and specificity specificity subset symptomatic patient Symptomatic patients Test tested viral infection with COVID-19 [DOI] 10.1136/jclinpath-2021-207516 PMC 바로가기 [Article Type] Article